mouse anti his tag primary antibody Search Results


95
Genecopoeia monoclonal mouse anti his
Monoclonal Mouse Anti His, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Sino Biological murine cd16 2
Murine Cd16 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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93
Cusabio anti his mouse monoclonal antibody
Anti His Mouse Monoclonal Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti his mouse monoclonal antibody - by Bioz Stars, 2026-03
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92
SouthernBiotech mouse antibodies
Mouse Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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93
SouthernBiotech anti his tag hrp
Anti His Tag Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
SouthernBiotech anti hexa his tag
Anti Hexa His Tag, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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92
Boster Bio anti his tag mouse monoclonal antibody
Anti His Tag Mouse Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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91
Cusabio anti his tag monoclonal mouse antibody
(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Anti His Tag Monoclonal Mouse Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti his tag monoclonal mouse antibody/product/Cusabio
Average 91 stars, based on 1 article reviews
anti his tag monoclonal mouse antibody - by Bioz Stars, 2026-03
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96
Qiagen penta his mouse 1 2000 qiagen 34660
(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Penta His Mouse 1 2000 Qiagen 34660, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
SouthernBiotech mouse anti histidine antibody
(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Mouse Anti Histidine Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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93
SouthernBiotech alexa fluor 647 conjugated anti mouse
(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Alexa Fluor 647 Conjugated Anti Mouse, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
SouthernBiotech anti mouse subclass specific antibody
(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .
Anti Mouse Subclass Specific Antibody, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse subclass specific antibody/product/SouthernBiotech
Average 93 stars, based on 1 article reviews
anti mouse subclass specific antibody - by Bioz Stars, 2026-03
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Image Search Results


(A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .

Journal: bioRxiv

Article Title: Evolutionary engineering a larger porin using a loop-to-hairpin mechanism

doi: 10.1101/2023.06.14.544993

Figure Lengend Snippet: (A, B) Western blots with the nitrocellulose membrane stained with Ponceau S and subsequently immunoblotted against various antibodies. (A) Western blot of different fractions of E. coli displaying the localization of the his-tagged recombinant proteins PorB WT and PorB 18 in the outer membrane. SurA, a periplasmic protein, indicates the localization of periplasmic proteins. Whole-cell fractions (WC), soluble (S), inner membrane and trapped periplasmic protein (IM/P), and outer membrane (OM) (B) Western blot displaying the susceptibility of proteins to Proteinase K under different conditions. His-tagged PorB WT (blue asterisks) and PorB 18 (green asterisks) show resistance to Proteinase K when not boiled but are partially susceptible when heat denatured. TolC (red asterisks) is used as a positive control as it shows a 5 kDa cleavage in the presence of proteinase K resulting in a 45 kDa cleavage product (red arrows) .

Article Snippet: The following antibodies were diluted as per manufacturer recommendation with TBST with 1% gelatin: THE Anti-His tag monoclonal mouse antibody (GenScript), anti- E. coli SurA polyclonal rabbit antibody (Cusabio), anti- E. coli BamA polyclonal rabbit antibody (Cusabio), anti- E. coli GroEL polyclonal rabbit antibody (Enzo Life Sciences), IRDye 800CW goat anti-mouse IgG secondary antibody (LI-COR Biosciences), IRDye 680RD donkey anti-rabbit IgG secondary antibody (LI-COR Biosciences). anti- E. coli TolC was purified from rabbit sera and diluted to 1:2500 with 1% gelatin in TBST.

Techniques: Western Blot, Membrane, Staining, Recombinant, Positive Control

(A) Single channel conductance of PorB 18 (blue) compared to PorB WT (green). (B) Box and whisker plot comparing the conductance of PorB WT (green) and PorB 18 (blue). ****: p-value <= 10 -4 (C) Graph shows comparison of experimental single-channel conductance of PorB WT with His-Tag (green) and without His-tag (light green), PorB 18 (blue), theoretical shingle-channel conductance of AlphaFold 18-stranded model (magenta) and 16-stranded model (cyan) of PorB 18 . (D) Channel conductance at 100 mV showing channel closing for individual subunits of PorB WT (green) and PorB 18 (blue). Shortened loop constructs collapse

Journal: bioRxiv

Article Title: Evolutionary engineering a larger porin using a loop-to-hairpin mechanism

doi: 10.1101/2023.06.14.544993

Figure Lengend Snippet: (A) Single channel conductance of PorB 18 (blue) compared to PorB WT (green). (B) Box and whisker plot comparing the conductance of PorB WT (green) and PorB 18 (blue). ****: p-value <= 10 -4 (C) Graph shows comparison of experimental single-channel conductance of PorB WT with His-Tag (green) and without His-tag (light green), PorB 18 (blue), theoretical shingle-channel conductance of AlphaFold 18-stranded model (magenta) and 16-stranded model (cyan) of PorB 18 . (D) Channel conductance at 100 mV showing channel closing for individual subunits of PorB WT (green) and PorB 18 (blue). Shortened loop constructs collapse

Article Snippet: The following antibodies were diluted as per manufacturer recommendation with TBST with 1% gelatin: THE Anti-His tag monoclonal mouse antibody (GenScript), anti- E. coli SurA polyclonal rabbit antibody (Cusabio), anti- E. coli BamA polyclonal rabbit antibody (Cusabio), anti- E. coli GroEL polyclonal rabbit antibody (Enzo Life Sciences), IRDye 800CW goat anti-mouse IgG secondary antibody (LI-COR Biosciences), IRDye 680RD donkey anti-rabbit IgG secondary antibody (LI-COR Biosciences). anti- E. coli TolC was purified from rabbit sera and diluted to 1:2500 with 1% gelatin in TBST.

Techniques: Whisker Assay, Comparison, Construct